His Tag Antikörper

Produktnummer ABIN7672606

Dieses Anti-His Tag-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von His Tag in WB, ELISA, IP und IF. Geeignet für .

Kurzübersicht für His Tag Antikörper (ABIN7672606)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: 1C4

Produktdetails anti-His Tag Antikörper

Verwendungszweck: 6*His, His-Tag antibody

Aufreinigung: Protein A+G purification

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: A synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:1000-1:10000, IP: 1:1000-1:10000, IF: 1:20-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu His Tag

Target: His Tag

Andere Bezeichnung: His-Tag

Substanzklasse: Tag

Hintergrund:

Synonyms: 6*His|6*His|His Tag|6histag|6XHis|HHHHHH|HIS|His Tag|HISTIDINE TAGGED

Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.